Effect of ultrasonication on selected enzymes from Neurospora crassa mycelia
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چکیده
منابع مشابه
Electron Microscopy of Neurospora crassa Mycelia
The fine structure of vegetative mycelia of the filamentous Ascomycete, Neurospora crassa, has been investigated by the standard techniques of electron microscopy. Addition of uranyl nitrate to the methacrylate-embedding medium minimized disruption of the specimens-an accident often observed in the preparation of microbial material. This report describes the presence of a chitinous polysacchari...
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Weibull (1953) demonstrated that the intact protoplasts are liberated from cells of Bacillus megaterium after digestion of the cell wall by the enzyme lysozyme, and that these structures persist and exhibit many of the activities of the whole cell provided they are maintained in solutions of appropriate osmotic pressure. Since that time free protoplasts have been obtained from many other gram-p...
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The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-h...
متن کاملEnzymes of deoxythymidine triphosphate biosynthesis in Neurospora crassa mitochondria.
Intact mitochondria of Neurospora crassa incorporate deoxythymidine 5'-monophosphate (dTMP) into deoxyribonucleic acid but not the label from (methyl-3H) deoxythymidine. Mitochondrial homogenates contain deoxythymidylate kinase (EC 2.7.4.9), deoxycytidylate aminohydrolase (dCMP deaminase) (EC 3.5.4.12), and thymidylate synthetase (EC 2.1.1b), but not thymidine kinase (EC 2.7.1.21) activity. dTM...
متن کاملInduction of lignocellulose-degrading enzymes in Neurospora crassa by cellodextrins.
Neurospora crassa colonizes burnt grasslands in the wild and metabolizes both cellulose and hemicellulose from plant cell walls. When switched from a favored carbon source such as sucrose to cellulose, N. crassa dramatically upregulates expression and secretion of a wide variety of genes encoding lignocellulolytic enzymes. However, the means by which N. crassa and other filamentous fungi sense ...
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 1963
ISSN: 1941-4765
DOI: 10.4148/1941-4765.2157